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<t>WNT</t> signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with <t>WNT</t> <t>inhibitor.</t> E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.
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<t>WNT</t> signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with <t>WNT</t> <t>inhibitor.</t> E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.
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<t>WNT</t> signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with <t>WNT</t> <t>inhibitor.</t> E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.
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<t>WNT</t> signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with <t>WNT</t> <t>inhibitor.</t> E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.
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<t>WNT</t> signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with <t>WNT</t> <t>inhibitor.</t> E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.
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WNT signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with WNT inhibitor. E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.

Journal: Advanced Science

Article Title: Large‐Scale Bioprinting of Human Epiblast‐Like Models Featuring Disc‐Shaped Morphogenesis and Gastrulation Events

doi: 10.1002/advs.202505340

Figure Lengend Snippet: WNT signaling pathway regulates the gastrulation‐stage characteristics of disc‐like structures. A) A representative plot of GSEA depicting WNT signaling pathway in the comparison of clusters on day6 versus day3. B) Dynamic expression wave of WNT signaling pathway related genes. C,D) Immunofluorescence co‐staining images of NANOG, BRACHYURY, SOX17 C) and VIMENTIN, OCT4 D) in clusters on day6 with WNT inhibitor. E) The developmental process of the epiblast layer in vivo and the evolution of the posterior epiblast tissues from hPSCs in vitro.

Article Snippet: In the experimental investigation of the regulatory effects of the WNT signaling pathway, 2.5 μM WNT inhibitor (MedChemExpress, HY‐12238) was incorporated into the self‐renewing medium from day 3 to day 6.

Techniques: Comparison, Expressing, Immunofluorescence, Staining, In Vivo, In Vitro